Hach Presence/Absence Broth/MUG Ampules
Features
- Easy-to-use test
- Color change to yellow or yellow brown indicates presence of coliform bacteria
- Screen drinking water samples for the presence of Total Coliforms and E. coli bacteria
- Expedited repair and warranty service
- Lifetime technical support
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Overview
The Hach P/A Broth with MUG allows simultaneous detection of total coliform bacteria and E. coli. In addition to the lactose and lauryl tryptose broths with bromcresol purple, this medium contains MUG reagent (4-methylumbelliferyl-b-D-glucuronide). The MUG reagent produces a fluorogenic product when hydrolyzed by glucuronidase (an enzyme specific to E. coli). MUG detects non-gasproducing (anaerogenic) strains of E. coli and works well when competitive organisms are present.
Incubation
Combine 100 mL of sample and P/A Broth with MUG, incubate for 24 hours and check for a color change and fluorescence. A yellow color indicates the presence of total coliforms. To detect E. coli, examine samples under long-wave ultraviolet (UV) light. Fluorescence indicates the presence of E. coli.
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